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Abstract Detail

Systematics Section/ASPT

Neubig, Kurt M. [1], Majure, Lucas [2], Abbott, Richard [3], Naczi, Robert F. C. [4], Judd, Walter [5], Whitten, M. Mark [6].

Preliminary phylogenetic relationships of Dichanthelium (Poaceae) as revealed through whole plastomes and nrDNA via genome skimming.

The genus Dichanthelium is distributed throughout the Americas, and Hawaii, with its highest species diversity in the eastern United States. Species delimitation within the genus is contentious, and low levels of sequence divergence result in poor resolution with conventional molecular markers. Using Illumina HiSeq sequencing technology, we “genome skimmed” plastomes without enrichment. Coverage of plastomes averaged approximately 30x across species sampled and the full nuclear ribosomal complex was recovered. Based on these data, Dichanthelium is monophyletic and relationships show better resolution than with nrITS alone, although problematic aspects of species level relationships remain.

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1 - Southern Illinois University, Plant Biology, 1125 Lincoln Dr., Carbondale, IL, 62901, USA
2 - Desert Botanical Garden, Department of Research, Conservation, and Collections, Phoenix, AZ, 85008, USA
3 - The New York Botanical Garden, 2900 Southern Blvd, Bronx, NY, 10458, USA
4 - The New York Botanical Garden, 2900 Southern Blvd., Bronx, NY, 0458-5126, USA
5 - University of Florida, Florida Museum of Natural History, Gainesville, FL, 32611, USA
6 - University Of Florida, Florida Museum Of Natural History, PO Box 117800, Gainesville, FL, 32611-7800, USA, 352/273-1964

Illumina HiSeq

Presentation Type: Oral Paper:Papers for Sections
Session: 50
Location: Salon 10/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 3:45 PM
Number: 50009
Abstract ID:563
Candidate for Awards:None

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