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Abstract Detail



Genomics / Proteomics

El-mezawy, Aliaa [1], Vidmar, John [1], Lange, Ralph [2].

Transcriptome analysis of Leptosphaeria maculans.

Blackleg caused by Leptosphaeria maculans is a fungal canker or dry rot disease of Brassica crops that leads to stem girdling and lodging. Severe damage can cause yield losses of 30%, resulting in farm gate losses of $152/acre, or over $177,000 for an average-sized canola farm in Alberta. Growing canola varieties that are moderately resistant or resistant to blackleg is recommended in areas with a high potential for virulent blackleg. The overall goal of our study is to develop novel sources of durable resistance to blackleg in canola via RNAi technology. The transcriptome analysis identified highly-expressed fungal genes that are essential for early infection by L. maculans. The transcriptome sequence reads from three blackleg isolates were compared against the current blackleg published sequence. The severely virulent (SV) isolate had 2,911,659 reads mapped, the virulent (V) isolate had 847,481 reads mapped, and the weakly virulent (WV) had 669,503 reads mapped. We identified a set of 54 transcripts from L. maculans, which are active during early stage of infection. The identified transcripts are the products of highly expressed genes during early infection of L. maculans in canola. Disruption of expression of one or more of these genes through plant biotechnology may create a novel source of blackleg resistance that can be deployed by plant breeders.


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1 - Alberta Innovates-Technology Futures, Biotechnology and Genomics, Hwy 16A & 75 Street, Vegreville, AB, T9C 1T4, Canada
2 - Alberta Innovates-Technology Futures, Feedstock Development and Microbial Products, Hwy 16A & 75 Street, Vegreville, AB, T9C 1T4, Canada

Keywords:
Blackleg
Transcriptome
Leptosphaeria maculans
RNAi.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGP004
Abstract ID:571
Candidate for Awards:None


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