Abstract Detail

Population Genetics

Andrew, Wilson [1], Elizabeth, Sheedy [2], Tom, May [3], Gregory, Mueller [4].

Population genetics of Laccaria sp. A: comparison of microsatellite and SNP data.

Laccaria species A is endemic to Tasmania and Victoria, Australia where it associates exclusively with Nothofagus cunninghamii. A recent population genetic study using eight mitochondria microsatellite markers demonstrated that its population structure was consistent with that of its ectomycorrhizal host and showed evidence of influence of past geological history (glacial refugia). There are a few recent studies that have compared the difference between microsatellite data and single nucleotide polymorphisms (SNPs) as population markers in animals and plants. However, no such evaluation has been published for fungi. We collected genomic SNP data using a genotyping by sequencing approach from samples of Laccaria sp. A that cover its geographic range consisting of material included in the microsatellite study plus additional samples. Analysis comparing microsatellite and SNP data were performed using F_st statistics and population structure using STRUCTURE to determine if SNPs provide a similar pattern to that documented with microsatellites. The SNP data for Laccaria sp. A was further evaluated using Diffusion Approximation for Demographic Information (DADI). This study is evaluating methods in fungal population genetics from multiple perspectives: the effectiveness of different molecular markers (microsatellites vs. SNPs) and the utility of different analytical tools (STRUCTURE vs. DADI).

1 - Chicago Botanic Garden, Plant Science and Conservation , 1000 Lake Cook Rd, Glencoe, IL, 60022, USA
2 - Marcroft Grains , Pathology, Grains Innovation Park, Horsham, Victoria, Australia
3 - Royal Botanic Gardens Melbourne, Mycology, Melbourne, Victoria, Australia
4 - Chicago Botanic Garden, Department Of Botany, 1000 Lake Cook Road, Glencoe, IL, 60022, USA

Keywords:
Agaricales Population Genetics
genotyping by sequencing
glacial refugia
 Nothofagus cunninghamii
DADI
structure
Australia.

Presentation Type: Oral Paper:Papers for Topics
Session: 71
Location: Salon 6/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 3:00 PM
Number: 71007
Abstract ID:585
Candidate for Awards:None