Abstract Detail

Genomics / Proteomics

Somalraju, Ashok [1], Alkhnajari, Aisha [1], Ghose, Kaushik [2], Main, David [3], Fofana, Bourlaye [4].

Use of NGS and Phenotyping for Developing EMS-Mutagenized Pre-breeding Potato lines Potentially Low in Anti-nutritional Factors.

Potato and its processed products are major components of human diets due to its nutritional properties. However, selection of valuable potato cultivars with important agronomic traits and low levels of anti-nutritional factors such as steroidal glycoalkaloids (SGAs), acrylamide formation precursors and browning, is a long a process. SGAs are secondary metabolites toxic to mammals but can play an important role in plant disease resistance. Acrylamide is a side product of the Maillard reaction and is potentially carcinogenic to humans. Breeding out anti-nutrients using traditional methods is labour intensive and time consuming and transgenic approaches are of concern to the public. In this study, an EMS mutagenesis approach coupled with EMS-induced SNP discovery in asparagine synthetase 1 (AS1), asparagine synthetase 2 (AS2), solanidine galactosyltransferase (SGT1), and solanidine glucosyltransferase (SGT2) and phenotyping were used for developing pre-breeding potato lines potentially low in anti-nutritional factors. EMS treatment induced visual phenotypic variations in plant morphology and tuber characteristics of the studied population. SNP calls from targeted amplicons resequencing using NGS within 1,750 EMS-treated and 140 non-treated lines identified 348 EMS-induced SNP mutations, of which 257 SNPs were located in exons of the four genes. The 257 EMS-induced exonic SNPs were detected in 133 independent EMS lines. Among the 257 EMS-induced exonic SNPs, 109 appeared to be sense mutations, 124 missense, and 24 nonsense SNPs. AS2 showed the highest EMS-induced mutations, with 232 SNPs representing 3.89 SNPs/100 bp, whereas SGT1 gene showed the lowest EMS-induced SNP number, with 13 SNPs and 0.77 SNPs/100 bp. Although preliminary, the data suggests the possibility of generating pre-breeding germplasm with altered biosynthetic pathways of anti-nutritional factors without rescue to transgenic approach.

1 - University of Prince Edward Island, Biology, 550 University Ave, Charlottetown, PE, C1A 4P3, Canada
2 - AAFC, 440 University Ave, Charlottetown, PE, C1A 4N6 , Canada
3 - AAFC, 440 University Ave, Charlottetown, PE, C1A 4N6 , Canada
4 - Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, PE, C1A 4N6, Canada

Keywords:
Pre-breeding Potato lines
EMS-Mutagenesis
Anti-nutrients
next generation sequencing
Phenotyping
Steroidal Glycoalkaloids
Acrylamide
Single nucleotide polymorphism
SGT1, SGT2, AS1 and AS2.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGP005
Abstract ID:598
Candidate for Awards:Genetics Section Poster Award,CSPB President's Award for Best Student Presentation,CSPB Travel Bursary