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Abstract Detail



Genomics / Proteomics

Heyduk, Karolina [1], Ray, Jeremy N. [2], Burrell, Nia [2], Lalani, Falak [2], Leebens-Mack, James H. [2].

CAM physiology and transcriptomics in the homoploid hybrid Yucca gloriosa.

Yucca gloriosa is a homoploid hybrid cross between Y. aloifolia and Y. filamentosa, a CAM and C3 species respectively. All three species are sympatric in the southeastern United States, overlapping along the coast and growing within beach sand dunes. Earlier floristic surveys indicated that Y. gloriosa utilizes an intermediate form of CAM photosynthesis. The intermediacy of CAM expression in these hybrids paralleled molecular work, which showed Y. gloriosa segregates for parental alleles in the wild. Despite CAM having evolved independently at least 35 times across angiosperms, still little is known about its evolution from the ancestral C3 state. Using the system found in Y. gloriosa, we explored differences between the CAM and C3 parents and their hybrid using a combination of physiological, anatomical, and transcriptomic approaches. We show the species differ significantly in their physiology and leaf anatomical characteristics, including the ability to utilize the CAM pathway under drought stress. We paralleled physiological work with RNA sequencing across the three species at six time points over a 24 hour diel cycle, comparing the same genotypes under well watered and drought stressed conditions. We find that core CAM-genes have expected expression patterns in the parental genotypes, and explore genes with correlated expression to the CAM pathway genes as possible regulators of CAM.


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1 - University Of Georgia, Plant Biology, 2502 Miller Plant Sciences, Athens, GA, 30602, USA
2 - University of Georgia, Department of Plant Biology, 2502 Miller Plant Sciences, Athens, GA, 30602, USA

Keywords:
Crassulacean acid metabolism
Photosynthesis
transcriptomics
comparative cenomics.

Presentation Type: Oral Paper:Papers for Topics
Session: 39
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 9:30 AM
Number: 39003
Abstract ID:599
Candidate for Awards:Margaret Menzel Award


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