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Abstract Detail


Waselkov, Katherine [1], Fehlberg, Shannon D. [2], Prather, L. Alan [3], Wright, Bethany [4], Ferguson, Carolyn J. [5].

Assessing allelic diversity in polyploid complexes of Phlox (Polemoniaceae) using next-generation amplicon sequencing.

Our interest in the primarily North American genus Phlox stems from widespread inter- and infraspecific ploidy level variation in the group. Flow cytometry, in combination with chromosome counts, has revealed that nearly 40% of species within the genus exhibit polyploidy (including some species with cytotypic variation). Our previous phylogenetic work with broad sampling in Phlox has shown widespread and non-trivial incongruence across the genus between trees reconstructed using nuclear genetic data (ITS) and chloroplast data, suggesting that the evolutionary history of Phlox has involved extensive reticulation, with and without genome doubling events. Based on these findings, we focus our current investigations on three closely related species complexes from the southwestern U.S. (P. amabilis, P. nana, and P. woodhousei), each of which contains diploid, tetraploid, and hexaploid populations. Using several low-copy nuclear markers, the origins of polyploid entities and the relationships among these entities and representatives from the broader genus are assessed. In order to sample multiple alleles within higher ploidy-level samples, we are using Illumina MiSeq next-generation technology and sequencing amplicons derived from PCR. Thus far, data have been collected for the low-copy nuclear gene isocitrate dehydrogenase B, using a combination of MiSeq and Sanger sequencing. Preliminary phylogenetic analyses show the utility of the MiSeq amplicon method for disentangling evolutionary relationships and taxonomic limits in a young group where recent polyploidy and hybridization are rampant.

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1 - Kansas State University, Division of Biology, Ackert Hall, Manhattan, KS, 66506, USA
2 - Desert Botanical Garden, 1201 N. Galvin Pkwy., Phoenix, AZ, 85008, USA
3 - Michigan State University, Department of Plant Biology, East Lansing, MI, 48824, USA
4 - Kansas State University, Division Of Biology, 116 Ackert Hall, Manhattan, KS, 66506, USA
5 - Kansas State University, Division Of Biology, Ackert Hall, Manhattan, KS, 66506-4901, USA

next generation sequencing

Presentation Type: Oral Paper:Papers for Topics
Session: 32
Location: Salon 11/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 10:45 AM
Number: 32011
Abstract ID:628
Candidate for Awards:None

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