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Abstract Detail

Population Genetics

Chiang, Yu-Chung [1], Yi, Ting-Shuang [2], Chen, Mei-Hui [3], Chung, Chia-Ying [4].

Genetic diversity, genetic divergence and phylogeographical patterns in Calocedrus based inferred by microsatellite data.

Yu-Chung Chiang and Ting-Shuang Yi : Equal contributors

The genus Calocedrus contains four relict species disjunctly distributed between East Asia and North America. Calocedrus decurrens Florin is native to western North America, C. macrolepis Kurz, and C. rupestris Aver., H.T. Nguyen & L.K. Phan are scattered throughout southwestern China to northern Indochina and Hainan Island, and C. formosana (Florin) Florin is endemic to Taiwan. According to Tertiary fossil evidence, Calocedrus had a wider distribution in whole Northern Hemisphere. In this study, we examined genetic diversity, genetic divergence and phylogeographical patterns in this genus based on microsatellite markers.Twenty polymorphic loci are used to evaluate genetic diversity among 627 samples from 27 populations of four Calocedrus taxa, including 10 populations from C. formosana, 14 populations from C. macrolepis, 2 populations from C. rupestris, and 4 populations fromC. decurrens. Genetic variation indices for 4 species base on 9 polymorphic loci, including the number of alleles (Na), the number of effective alleles (Ne), and the observed and expected heterozygosity (Ho and He), are estimated. Na ranged from 3 to 11, 3 to 20, 2 to 12, and 0 to 6(means:6.900, 8.050, 5.000, and 3.650) in C. formosana, C. macrolepis, C. rupestris, and C. decurrens, respectively. Ne varied from 1.262 to 3.640, 1.154 to 3.546, 1.131 to 5.354, and 0 to 4.122 (means: 2.042, 2.277, 2.656, and 2.510) in the four Calocedrus species. Ho and He range from 0 to 0.943 and 0.207 to 0.633, respectively, in C. formosana, 0 to 0.981 and 0.133 to 0.709 in C. macrolepis, 0 to 0.939 and 0.116 to 0.813 inC. rupestris, and 0 to 0.467 and 0 to 0.737 in C. decurrens. The result of principal coordinates analysis (PCoA) showed that high genetic divergence between species except between C. macrolepis and C. rupestris. The analysis of molecular variance (AMOVA) displayed 18.655% genetic variation accumulated between species and also show high levels of genetic differentiation (φST = 0.187). AMOVA revealed most microsatellite DNA variants resided among species and populations, a pattern was also supported by clustering with microsatellite STRUCTURE analyses. Significant genetic structuring within four Calocedrus species was detected among three geographical regions (western North America, mainland Asia, and Taiwan); a phylogeographical pattern is likely attributable to past fragmentations. Furthermore, the low likelihood of recurrent gene flow due to geographical subdivision, all results of above analyses revealed species divergence, reflecting a history of speciation caused by geographical separation during the Quaternary glaciation.

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1 - National Sun Yat-Sen University, Department Of Biological Sciences, No. 70, Lienhai Road,, Kaohsiung, Taiwan, N/A, 80424, Taiwan
2 - Kunming Institute of Botany, Chinese Academy of Sciences, Germplasm Bank of Wild Species in Southwest China, 132# Lanhei Road, Heilongtan, , Kunming, Yunnan, 650201, P.R. China
3 - Forestry Bureau, Council of Agriculture, Executive Yuan, , Conservation Divisions, No. 2, Hangchou South Road, Section 1,, Taipei, 100, Taiwan
4 - National Sun Yat-Sen University,, Department Of Biological Sciences,, No. 70, Lienhai Road, , Kaohsiung,, 80424, Taiwan

genetic diversity
population genetics

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPG003
Abstract ID:637
Candidate for Awards:None

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