Abstract Detail

Genomics / Proteomics

Adhikary, Dinesh [1], Deyholos, Michael [1].

Transcriptome profiling of Amaranthus hypochondriacus..

Grain amaranth has been cultivated for thousands of years in Central and South America. It provides a complete source of protein including lysine and methionine. It has high tolerance to biotic and abiotic stress, and has potential to thrive in varying environmental conditions. Due to its exceptional physiological, functional, and nutritional traits, it has promise as a new crop. Molecular studies are rapidly increasing for grain amaranths; however, transcriptome information is not adequate. So to develop more transcript information, eight-different tissues (normal flower, leaf, root, and stem, drought-stressed leaf, root, and stem, immature seeds, maturing seeds, and green cotyledons) were sequenced using Hiseq Illumina platform and produced 352,557,994 raw reads, and that evolved into 97,741 contigs with N₅₀ contig length 1705 bp. Minimum contig length was 350 and maximum length was 12841. A total of 50.40% of these contigs has significant (e-value 10^-10) hits to Arabidopsis protein coding sequences matching 14,102 unique proteins. These results demonstrated that Illumina platform can help discover broad range of genes involved in the developmental and stress related genes in grain amaranths.

1 - The University of British Columbia, Biology, 1177 Research Road, Kelowna, BC, V1V 1V7, Canada

Keywords:
Transcriptome
Amaranthus .

Presentation Type: Oral Paper:Papers for Topics
Session: 48
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 4:15 PM
Number: 48011
Abstract ID:65
Candidate for Awards:Margaret Menzel Award