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Abstract Detail

Symbioses: Plant, Animal, and Microbe Interactions

Kamino, Leila. N [1], Gulden, Robert H. [2].

Linking crop species to soil DNase activity and microbial biomass.

In addition to abiotic properties of soil, plant species have profound influence on microbial community structure and function. This is largely because plants are the main providers of specific carbon and energy sources that are readily available to soil microbes. Among these energy sources, extracellular plant DNA (eDNA) serves as a source of nutrients resulting to a change in community structure and function. The rate of this nutrient availability is determined by the presence of nuclease producing bacteria in the vicinity available to restrict the eDNA. Extracellular DNases produced by soil microbes have an impact on the persistence of eDNA which is important for horizontal gene transfer. There is paucity of information on how plant species composition influence soil microbial functions such as DNase activity. Therefore, the objective of this study was to assess how different crops influence soil microbial DNase activity and biomass over time. A greenhouse experiment was conducted using four crop species; alfalfa, canola, soybean and wheat, which were sown into field soils collected in Carman, Manitoba, and unplanted pots were used as controls. Pots were leached at different growth stages and the DNase activity in the leachate assessed using the DNA-methyl green plate assay and the DNA-methyl green spectrophotometric assay. Crop species significantly altered DNase activity, total culturable DNase producing bacteria and total culturable bacterial populations of the soil. When different from the control, soybean increased the DNase activity and other parameters in soybean were similar to the unplanted control and when different were greater than in the unplanted control. Canola, on the other hand, consistently showed the lowest DNase activity and reduced values in all other measured parameters. The results indicate that plant species have a large impact on below-ground function and the functional composition of the soil microbial community.

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1 - University of Manitoba, Plant Science, 222 Ag Building, 66 Dafoe Road, Winnipeg, MB, R3T 2N2, Canada
2 - Univeristy of Manitoba, Plant Science, 222 Ag Building, 66 Dafoe Road, Winnipeg, MB, R3T 2N2, Canada

Agricultural Crops
Soil Microbe
Enzyme activity.

Presentation Type: Oral Paper:Papers for Topics
Session: 36
Location: Salon 5/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 11:45 AM
Number: 36015
Abstract ID:677
Candidate for Awards:Economic Botany Section best poster

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