| Abstract Detail
Systematics Section/ASPT Martine, Chris [1], Jordon-Thaden, Ingrid [1], Roche, Morgan [2], Hayes, Daniel [2], Tank, David [3]. Transcriptome-mined introns offer new understandings of phylogenetic relationships among Australian bush tomatoes (Solanum) within the context of the Old World spiny solanums. Relationships among the spiny solanums of Australia have defied phylogenetic resolution for some time, especially the dioecious and andromonoecious taxa of the northern subarid tropics. Previous work using the ITS and trnK/matK gene regions helped define a number of clades, but left much to be desired relative to both the relationships among those clades and among the closely-allied species within them. For this study we analyzed data generated from ca. 50 Solanum species from Australia, the rest of the Old World, and the New World in an attempt to clarify relationships among the Australian taxa and to further explore the phylogeny of Old World spiny solanums. Phylogenetic inference was made from select unknown intronic regions mined from six transcriptomes from the 1000 Plants (1KP) project. 173 intronic regions were mined from the transcriptomes using MarkerMiner 1.0, and primer pairs for these regions were designed with the Primer3 plugin in Geneious. Seventeen of these primer pairs were validated and used for phylogenetic inference. The results shed new light on the origin of functional dioecy in Australian Solanum, clarify relationships among a number of rare and unusual species, aid in the recognition of new species, offer insight into recent speciation events, and inform efforts to develop conservation strategies for a number of species endemic to northern Australia’s Sandstone Country.
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1 - Bucknell University, Biological Sciences, 203 Biology Building, Lewisburg, PA, 17837, USA, 570/577-1135
2 - Bucknell University, Biology C/o C. Martine, 1 Dent Drive, Lewisburg, PA, 17837, USA
3 - University Of Idaho, Peabody Museum Of Natural History, 875 Perimeter Dr MS 3051, Moscow, ID, 83844-3051, USA
Keywords:
Solanum
solanaceae
Leptostemonum
dioecy
andromonoecy
Australia
MarkerMiner
transcriptomics
breeding system
reproductive biology.
Presentation Type: Oral Paper:Papers for Sections
Session: 60
Location: Salon 2/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 9:00 AM
Number: 60005
Abstract ID:685
Candidate for Awards:None |
