| Abstract Detail
Population Genetics Roche, Morgan [1], Jordon-Thaden, Ingrid [2], Hayes, Daniel [1], Boni, Alexandra [3], Martine, Chris [2]. Population genetics of two study systems using ddRADtag and HiSeq Illumina. This study employs ddRADtag with double digestion followed by HiSeq Illumina sequencing, to compare genetic variation within and among regionally sympatric populations in two separate study systems: Australian Solanum spp (Solanaceae). and North American Cordillera Draba oligosperma (Brassicaceae). The six spiny Australian species are: S. asymmetriphyllum, S. clarkiae, S. cowiei, S. dioicum-Tanami, S. echinatum, and S. sejunctum. For this Solanum study 21 populations and approximately 400 individuals were analyzed. For Draba oligosperma, 29 populations of 20 individuals per population were included. Detailed information about the a priori choosing the pair of restriction enzymes and the further laboratory steps to prepare the ddRADtag libraries will be presented. Various levels of multiplexing individuals were tested for sequencing depth. Log in to add this item to your schedule
1 - Bucknell University, Biology C/o C. Martine, 1 Dent Drive, Lewisburg, PA, 17837, USA 2 - Bucknell University, Biological Sciences, 203 Biology Building, Lewisburg, PA, 17837, USA, 570/577-1135 3 - Bucknell University, Biology Dept, Biology Bldg 203, Lewisburg, PA, 17837, USA
Keywords: ddRADtag Brassicaceae solanaceae population genetics restriction enzymes.
Presentation Type: Poster:Posters for Topics Session: P Location: Hall D/The Shaw Conference Centre Date: Monday, July 27th, 2015 Time: 5:30 PM Number: PPG005 Abstract ID:692 Candidate for Awards:Genetics Section Poster Award |