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Abstract Detail



Population Genetics

Roche, Morgan [1], Jordon-Thaden, Ingrid [2], Hayes, Daniel [1], Boni, Alexandra [3], Martine, Chris [2].

Population genetics of two study systems using ddRADtag and HiSeq Illumina.

This study employs ddRADtag with double digestion followed by HiSeq Illumina sequencing, to compare genetic variation within and among regionally sympatric populations in two separate study systems: Australian Solanum spp (Solanaceae). and North American Cordillera Draba oligosperma (Brassicaceae). The six spiny Australian species are: S. asymmetriphyllum, S. clarkiae, S. cowiei, S. dioicum-Tanami, S. echinatum, and S. sejunctum. For this Solanum study 21 populations and approximately 400 individuals were analyzed. For Draba oligosperma, 29 populations of 20 individuals per population were included. Detailed information about the a priori choosing the pair of restriction enzymes and the further laboratory steps to prepare the ddRADtag libraries will be presented. Various levels of multiplexing individuals were tested for sequencing depth.


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1 - Bucknell University, Biology C/o C. Martine, 1 Dent Drive, Lewisburg, PA, 17837, USA
2 - Bucknell University, Biological Sciences, 203 Biology Building, Lewisburg, PA, 17837, USA, 570/577-1135
3 - Bucknell University, Biology Dept, Biology Bldg 203, Lewisburg, PA, 17837, USA

Keywords:
ddRADtag
Brassicaceae
solanaceae
population genetics
restriction enzymes.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPG005
Abstract ID:692
Candidate for Awards:Genetics Section Poster Award


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