Abstract Detail

Reproductive biology

Hayes, Daniel [1], Jordon-Thaden, Ingrid [2], Boni, Alexandra [3], Sharbel, Timothy [4], Koch, Marcus [5], Martine, Chris [2].

Flow cytometric seed screen of the apomictic alpine mustard, Draba oligosperma Hook., from the North American Cordillera.

Draba oligosperma Hook. (Brassicaceae) has a unique distribution pattern and an unusual mode of apomixis. Preliminary data from one population showed this species to be apomictic, but historical herbarium sheets containing pollen suggests it is sexual in parts of its distribution. To determine if the species is apomictic throughout its distribution, seed collections from California, Nevada, Utah, Idaho, Montana, Wyoming, and the Yukon were made during 2012, 2013, and 2014 field seasons. A total of 29 populations were sampled with ~30 individuals per population. For the seed screen, we have analyzed approximately 50% of the samples (~4350 seeds). Using high throughput flow cytometric seed screening (FCSS), we determined the stability of apomictic reproduction for this species. The unique and large distribution of this species will allow for the role of apomixis in speciation to be better understood.

1 - Bucknell University, Biology C/o C. Martine, 1 Dent Drive, Lewisburg, PA, 17837, USA
2 - Bucknell University, Biological Sciences, 203 Biology Building, Lewisburg, PA, 17837, USA, 570/577-1135
3 - Bucknell University, Biology Dept, Biology Bldg 203, Lewisburg, PA, 17837, USA
4 - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Cytogenetics and Genome Analysis, Apomixis, Gatersleben, 06466, Germany
5 - Heidelberg University, COS, Diversity and Plant Systematics, Im Neuenheimer Feld 345, Heidelberg, Germany

Keywords:
apomixis
Draba
Brassicaceae
population genetics
Polyploidy
Flow Cytometry Seed Screen (FCSS).

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRP006
Abstract ID:693
Candidate for Awards:None