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Abstract Detail



Horticulture

Arigundam, Usha [1], Marshall, H.Dawn [1], Debnath, Samir.C [1].

Scaling up lingonberry micropropagation in liquid media using a bioreactor system.

The culture growth rate during micropropagation varies based on the physical nature of the medium. Although lingonberry explants are easily seen and recovered on semi-solid media, the rates of growth are slow because of the toxic exudates from explants. Micropropagation on a semi-solid medium is time consuming and labor intensive. Use of liquid media could result in faster rates of growth due to greater surface area of the explant in contact with medium, and the medium makes nutrients available to plant tissue more efficiently than semi-solid media. In this study, three in vitro culture systems differing in physical properties of the growing medium were used to investigate their influence on number of days to callus formation, callus survival percentage and number of shoots. The experiment was set up using commercial lingonberry cultivars Sanna and wild clones NL1 and NL2. The systems under comparison were semi-solid media and two liquid media, one with a temporary bioreactor (RITAR) and the other with stationary immersion (GrowtekTM), all three containing thidiazuron (TDZ) and zeatin at different concentrations. The formation of condensed organized structures, days to callus formation and a good number of shoots were observed earlier in liquid media than in semi-solid media. Temporary immersion performed better than stationary immersion for shoot regeneration. The major problem observed in stationary immersion was hyperhydricity, which is a serious physiological disorder that can be eliminated by adjusting the immersion times. Increased shoot vigor in temporary immersion in a bioreactor system (RITAR) indicates immersion time as the most decisive parameter for system efficiency. These results suggest that the micro environmental conditions in liquid media with a temporary bioreactor provide rapid and efficient plant propagation compared stationary liquid or semi-solid media.


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1 - memorial university of newfoundland, 232 Elizabeth Avenue, st john's, NL, A1B 3X9, Canada

Keywords:
Micropropagation
semi-solid media
stationary immersion
temporary immersion
Lingonberry.

Presentation Type: Oral Paper:Papers for Topics
Session: 14
Location: Salon 19/20/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:15 PM
Number: 14004
Abstract ID:724
Candidate for Awards:None


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