| Abstract Detail
Cell biology Ruan, Yuan [1], Wasteneys, Geoffrey O. [2]. The CLASP microtubule-associated protein modulates and is also a direct transcriptional target of the brassinosteroid signalling pathway during root apical meristem development. In plants, the root meristem is required to provide new cells for sustaining root growth and is regulated by brassinosteroids (BRs) and microtubules. Here, we describe a mechanism wherein BR controls meristem size through microtubules. We show that BR promotes precocious exit of meristematic cells to elongation by CLASP-mediated cortical microtubule reorientation. BRASSINAZOLE-RESISTANT 1 and 2 (BZR1 and BZR2/BES1), the two key transcription factors in the BR signalling pathway, directly target and repress CLASP expression. BR also leads to altered CLASP distribution. clasp-1 null mutants display defects in cortical microtubule pattern and smaller meristems, reminiscent of BR-treated plants. We also find a facilitation effect of CLASP and microtubules on the recycling of the surface receptor, BRASSINOSTEROID-INSENSITIVE 1 (BRI1) to the plasma membrane. clasp-1 mutants show enhanced vacuolar targeting of BRI1 and an impaired BR response. The repression on CLASP buffers BR signalling in meristem maintenance.
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1 - University of British Columbia, Botany, 3529-6270 university blvd, vancouver, bc, v6t1z4, Canada
2 - University of British Columbia, 3529-6270 university blvd, vancouver, bc, v6t1z4, Canada
Keywords:
Arabidopsis
Microtubule-associated protein
CLASP
Brassinosteroid
BRI1.
Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PCL002
Abstract ID:746
Candidate for Awards:CSPB President's Award for Best Student Presentation |
