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Abstract Detail



Cell biology

Selvaraj, Kumarakurubaran [1], Bonham-Smith, Peta [1].

Role of ribosome assembly factor HRR25 in Arabidopsis growth and development.

Ribosomes are ubiquitous-enzymatic nanomolecular machines in all living cells, driving translation of the genome into the proteome. Ribosome biogenesis is fundamental to cellular life and is coupled to cellular homeostasis and growth rate. Improper ribosome assembly could result in nonfunctional proteins. In yeast, generating a functional ribosome requires numerous rRNA processing events in coordination with 200 ribosome biogenesis factors (RBFs). Ribosome biogenesis has been extensively studied in bacteria, yeast and mammals, however, little is known in plants. In yeast ribosomal biogenesis, HRR25 is an essential gene coding for a casein kinase1-like (CK1L) protein. Hrr25p interacts with Ltv1p-Enp1 and regulates the incorporation of RPS3 and RPS10 that line, the mRNA entry channel of the 40S small subunit, thereby controlling key maturation events. Hrr25p also phosphorylates Tif6p an essential 60S RBF essential for subunit association. A Hrr25p-Nup53p (nuclear pore complex) interaction suggests that the mobility of Hrr25p during cell division may effect the cell cycle. A total of 14 CK1L isoforms (1-13, 9α- β) have been reported in Arabidopsis thaliana as plasmodesmata-associated kinases. Phylogenetic analysis of these CK1L isoforms shows, five among the 14 closely cluster with yeast Hrr25p. A role for AtHRR25 in ribosome biogenesis has yet to be demonstrated. Through: i) yeast null mutant complementation, ii) yeast 2-hybrid analysis, along with tandem-affinity purification chromatography, to establish the AtHRR25 in planta interactome, iii) phenotyping AtHRR25 T-DNA insertion lines, iv) cellular localization of AtHRR25, v) construction of RNAi and over-expression paralogue lines and vi) developmental expression analysis of each paralogue, we will establish the role(s) of AtHRR25 in plant ribosome biogenesis.


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Related Links:
Eukaryotic ribosome biogenesis
HRR25 in Ribosome biogenesis


1 - University of Saskatchewan, Biology, 127-112 Science place, Saskatoon, SK, S7N5E2, Canada

Keywords:
Ribosome biogenesis
HRR25
Arabidopsis.

Presentation Type: Oral Paper:Papers for Topics
Session: 58
Location: Salon 17/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 10:15 AM
Number: 58009
Abstract ID:773
Candidate for Awards:CSPB President's Award for Best Student Presentation,CSPB Travel Bursary


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