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Abstract Detail



Genomics / Proteomics

Sinn, Brandon T. [1], Sedmak, Dylan [2], Kelly, Lawrence [3], Freudenstein, John [4].

Not all magnoliid genomes are “fossils.”.

The magnoliids are commonly thought of as relictual holdouts whose organellar genomes have been to-date characterized as unrearranged and pleisiomorphic with respect to length, and gene and syntenic block makeup. However, our sequencing of plastid and mitochondrial genomes in Asarum (Aristolochiaceae) has shown that the genomes of this derived magnoliid lineage do not fit this model. Using a combination of de novo and iterative assembly of Illumina MiSeq 300 bp and HiSeq 100bp paired-end reads, we have constructed mitochondrial contigs from Saruma henryi and six Asarum species (A. canadense, Asarum sect. Asarum; A. delavayi, Asarum sect. Longistylis; A. epigynum, Asarum sect. Geotaenium; A. megacalyx, Asarum sect. Heterotropa; A. minus, Asarum sect. Hexastylis; and A. sieboldii, Asarum sect. Asiasarum). Similarly to the plastomes of these species, the mitochondrial genome is more divergent from the “fossilized” mitochondrial genome of Liriodendron tulipifera (Magnoliaceae) than would be expected given the currently published characterizations of organellar genome evolution in the magnoliids. Of more interest than simple rearrangement, which is expected in the mitochondrial genome, the modification, loss, and gain of syntenic blocks in similar fashion to derived lineages elsewhere in the angiosperms are apparent. Furthermore, approximately twenty percent of annotated RNA editing sites in the Liriodendron mitochondrion have been lost in Asarum minus. Our work suggests that the same trends, such RNA editing site loss, chloroplast to mitochondrial transfer, and mitochondrial genome expansion, have impacted the mitochondrial genomes of Asarum species as those that have shaped the mitochondrial genomes of the eudicots and monocots.


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1 - Ohio State University, Evolution, Ecology and Organismal Biology, 1315 Kinnear Road, Columbus, OH, 43212, USA
2 - Ohio State University
3 - The New York Botanical Garden, Bronx, NY, 10458
4 - Ohio State University, Museum Of Biological Diversity, 1315 Kinnear Rd, Columbus, OH, 43212-1157, USA

Keywords:
Asarum
Saruma
Magnoliid
mitochondrion
mitochondrial genome.

Presentation Type: Oral Paper:Papers for Topics
Session: 39
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 11:30 AM
Number: 39010
Abstract ID:778
Candidate for Awards:Margaret Menzel Award


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