Abstract Detail

Symbioses: Plant, Animal, and Microbe Interactions

Hirsch, Ann M. [1], Maymon, Maskit [2], Martínez-Hidalgo, PIlar [2], Tran, Stephen [3], Ice, Tyler [4], Craemer, Karena [4], Anbarchian, Teni [4], Hwang, Lin H. [5], Faull, Kym F. [5].

Plants do not live alone.

Interest in the human microbiome has focused attention on the phytomicrobiome, which like its human counterpart, protects the plant from disease, provides nutrients, and influences plant growth. We have been studying Bacillus simplex, a recently described plant growth promoting bacteria (PGPB), which has positive effects on legume growth, especially when coinoculated with alpha- and beta-rhizobia. B. simplex inhabits both the rhizosphere and the endosphere. We queried the B. simplex 30N-genome for genes that encode products that could positively influence plant growth, such as genes for hormone biosynthesis, volatile production, siderophores, phosphate solubilization, etc., by comparing B. simplex genome sequences to those of known PGPB. Although B. simplex 30N-5 was shown earlier to be positive for auxin production by the Salkowski test, it does not have a complete auxin biosynthetic pathway, and chemical analysis determined that IAA was not produced. However, this species possesses genes for polyamine synthesis and produces both spermidine and putrescine, which can positively affect plant growth. Genes for the synthesis of the typical antibiotics produced by bacilli were also not found in the B. simplex genome, but genes for the synthesis of a previously uncharacterized cyclic imine were detected. The exact mechanisms whereby these novel gene products influence plant growth and how this bacillus species interacts with rhizobial species are goals for future study. Funded by NSF IOS 1201735 and the Shanbrom Family Foundation and in part, by the US Dept. of Energy Joint Genome Institute, supported by Contract No. DE-AC02-05CH11231.

1 - University Of California Los Angeles, Dept Of Mol, Cell, & Devel Bio, 621 Charles Young Dr., South, Los Angeles, CA, 90095-1606, USA
2 - University of California-Los Angeles, Molecular, Cell and Developmental Biology, Los Angeles, CA, 90095-1606, USA
3 - University of California-Los Angeles, Bioinformatics, Los Angeles, CA, 90095
4 - University of California-Los Angeles, Molecular, Cell and Developmental Biology, Los Angeles, CA, 90095-1606
5 - University of California-Los Angeles, Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior and Department of Psychiatry and Biobehavioral Sciences, Dav, Los Angeles, CA, 90095-1606
6 - University of California-Los Angeles, Pasarow Mass Spectrometry Laboratory, Semel Institute for Neuroscience and Human Behavior and Department of Psychiatry and Biobehavioral Sciences, Dav, Los Angeles, CA, 90095-1606

Keywords:
none specified

Presentation Type: Oral Paper:Papers for Topics
Session: 51
Location: Salon 5/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 4:45 PM
Number: 51013
Abstract ID:824
Candidate for Awards:None