Abstract Detail

Phylogenomics

Johnson, Matthew [1], Wickett, Norm [2], Medina, Rafael [3], Liu, Yang [3], Devos, Nicolas [4], Shaw, Jonathan [5], Goffinet, Bernard [6].

Phylotranscriptomic insights into the radiation of mosses: gene family expansions and paleopolyploidy.

Rapid radiations— early bursts in the phylogeny connected by short internal branches—are found in many major lineages of land plants, and leave a signature of diversification in the genome, such as whole genome duplication (WGD), gene family expansion, or horizontal gene transfer (HGT). Phylogenomic techniques can be applied even in non-model organisms by comparing transcriptomes from many species to determine whether genomic events are associated with major lineages. In the pleurocarpous mosses (approx. 4200 species), a single radiation ca. 130 mya is associated with a derived, frequently epiphytic, growth form and an early burst of diversification that coincides with the rise of the angiosperms. We compared twenty transcriptomes of pleurocarpous mosses from the order Hypnales along with five transcriptomes of acrocarpous mosses and the proteome of the model moss Physcomitrella patens. Using a bioinformatics pipeline, we filtered transcripts according to similarity to known plant proteins, and organized them into orthologous gene families with a phylogenetic clustering approach. We found evidence of several hundred gene family expansions associated with the expansion of the pleurocarpous mosses, and evidence that several reported HGT events are much more recent events than previously thought.

1 - Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, ILLINOIS, 60022, United States
2 - Chicago Botanic Garden, 1000 Lake Cook Rd., Apt 2, Glencoe, IL, 60022, USA
3 - University of Connecticut, Evolution and Ecology, 75 North Eaglevlle Rd, Storrs, CT, 06269, United States
4 - Duke University, Biology, Box 90338, Durham, NC, 27705, United States
5 - 130 Science Drive, Box 90338, Durham, NC, 27708, USA
6 - University Of Connecticut, Department Of Ecology & Evolutionary Biology, 75 N. Eagleville Road, U-3043, Storrs, CT, 06269-3043, USA

Keywords:
Bryophyte
Transcriptome
gene family
orthologs.

Presentation Type: Oral Paper:Papers for Topics
Session: 32
Location: Salon 11/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 11:30 AM
Number: 32014
Abstract ID:877
Candidate for Awards:Margaret Menzel Award