Abstract Detail

Biotic and abiotic stress

Caputlu, Suleyman [1], Ucarli, Cuneyt [1], Yoruk, Emre [1], Karaer, Semian [1], Gurel, Filiz [1].

Allelic diversity in Hordeum spontaneum at the Dhn3 locus: a major dehydration stress-inducible gene in barley.

Dehydrins are a conserved gene family in plants that induced in late stages of embryo development and by dehydration stress, salinity, low temperature and abscisic acid (ABA) treatments. Dhn3 allelic diversity in Hordeum spontaneum (C.Koch) Thell which is widely distributed in Turkey is unknown. In this study, wild barley populations originated from different geographic regions of Turkey were collected and investigated for Dhn3 sequence diversity. Total nucleotide polymorphism (π) within the Dhn3 gene was calculated as 0.00636. When intragenic regions were analyzed, introns and the K₁ segment were found to have the highest polymorphism rates as 0.01247 and 0.01354, respectively. Dhn3 gene in H. spontaneum was YSK₂ type and thus similar to H. vulgare. However, some of the accessions carried non-synonymous changes led to different patterns of Y, S and K segments that previously reported in literature. A total of 32 mutations including 31 single nucleotide polymorphisms (SNPs) and one indel mutation were detected in 674-bp gene region. In comparison to cv. Dicktoo, only six of 21 SNPs identified in exon regions caused synonymous mutations in the amino acid sequences. It was also remarkable that some amino acid variations led to changes in hydrophobicity and Ip of the resulted polypeptides that having only few secondary structures. The nature of the allelic diversity and bioinformatic analyses of H. spontaneum accessions allowed for predictions to understand intrinsic disordered structure of Dhn3 and putative interaction sites with the other ligands.

1 - Istanbul University, Department of Molecular Biology and Genetics, Science Faculty, Vezneciler, Istanbul, 34134, Turkey

Keywords:
genetic diversity
Dhn3
drought.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PBA001
Abstract ID:89
Candidate for Awards:None