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Abstract Detail



Host/Plant Pathogen Interactions and Plant Health Management

Hossain, Md. Musharaf [1], Sheoran, Inder S. [1], Stewart, Chad [1], Wei, Yangdou [2], Bonham-Smith, Peta [1].

Identification of Plasmodiophora brassicae genes expressed during infection of Arabidopsis thaliana Col-0.

Plasmodiphora brassicae is a devastating obligate biotrophic pathogen that causes clubroot disease in Brassicas. Currently there is limited information available describing the pathogenicity and host-pathogen interaction mechanisms for this pathogen; only a few pathogenesis related genes have so far been identified. Identification and functional characterization of P. brassicae effector genes is essential for designing effective control strategies against this pathogen. In this study, the expression of 79 predicted putative secretory protein coding genes were studied at different time points; 0, 2, 5, 7, 14, 21 and 28 days post infection (DPI) of A. thaliana Col-0 with P. brassicae pathotype 3. RT-PCRs, using primers specific for each of the 79 clones, showed that 27 clones were not expressed in resting spores of P. brassicae but were expressed at later stages of infection. These genes may be associated with pathogenicity and disease progression. A number of the 79 genes showed significant up- or down-expression over the time course of this study. Of the 79 genes, 45 were expressed at DPI5 and their expression continued throughout the later stages of the time course and disease progression. The distinct expression patterns of some of the genes specific to certain infection stages suggest a relative importance during primary and/or secondary infection with P. brassicae. Future research focus will be on identifying the functions of those highly expressed and regulated ‘unknown genes’ for which we currently have no available annotation.


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1 - University of Saskatchewan, Department of Biology, 127-112-Science Place, Saskatoon, SK, S7N 5E2, Canada
2 - University of Saskatchewan, Department of Biology, 226-112-Science Place, Saskatoon, SK, S7N 5E2, Canada

Keywords:
Clubroot
P. brassicae
Resting spore
Biotrophic
Effectors
RT-PCR.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA005
Abstract ID:910
Candidate for Awards:CSPB President's Award for Best Student Presentation,CSPB Travel Bursary


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