Abstract Detail

Genetics Section

Brar, Gurcharn [1].

Is Puccinia striiformis f. sp. tritici population in western Canada is really clonal?

Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Selection pressure on the pathogen population may result in a rapid shift to races virulent on wheat genotypes with specific resistance genes. For successful stripe rust management, it is important to characterize the population structure of the pathogen. The purpose of this research was to determine the genotypic diversity of the pathogen population. Forty eight isolates of Pst were used for this study including four old isolates (collected before 2000) and 44 new isolates (collected after 2000). Whole genome sequencing of pathogen isolates was conducted, using the Illumina HiSeq2500 platform and polymorphisms were assessed by high quality single nucleotide polymorphism (SNP) variants. Diversity at the genome level was not observed to be related to geographic location or virulence phenotypes of the isolates. The SNP data revealed four sub-populations in the western Canadian Pst population. Genomic analyses of 48 Pst isolates did not reveal any strong relationship of the four sub-populations with their origin or year of collection. Signs of recombination were detected in the Pst population in western Canada. Genomic analyses differentiated isolates showing signs of recombination from those that did not.

1 - University of Saskatchewan, Plant Sciences, 51 Campus Dr., Agriculture Building, Saskatoon, SK, S7N 5A8, Canada

Keywords:
none specified

Presentation Type: Oral Paper:Papers for Sections
Session: 17
Location: Salon 15/16/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:30 PM
Number: 17005
Abstract ID:916
Candidate for Awards:Margaret Menzel Award