| Abstract Detail
Host/Plant Pathogen Interactions and Plant Health Management Bakkeren, Guus [1], Linning, Rob [1], Joly, David [2], Khalil, Hala [3], Wang, Xiben [4], Panwar, Vinay [5], Fellers, John [6], Szabo, Les [7], Hulbert, Scot [8], Chen, Xianming [9], Hamelin, Richard C. [10], McCallum, Brent [4], Cuomo, Christina [11], Saville, Barry [12]. Comparative genomics of rust fungi to probe wheat interactions; the road to crop protection. Rust fungi cause devastating plant diseases on cereal crops. We have previously sequenced reference genomes of Puccinia graminis f.sp. tritici (Pgt, causing stem rust), P. striiformis f. sp. tritici (Pst, stripe rust) and P. triticina (Pt, leaf rust; http://www.broadinstitute.org). Comparative analyses of predicted proteomes reveal 3,728 (Pt), 4,376 (Pgt) and 6,567 (Pst) species-specific proteins, with over 2,244 orthologs specific to all three cereal rusts; comparisons to related poplar and flax rust genomic resources serve to identify specific pathogenicity genes. We also report on the re-sequencing of 30 Canadian Pt races and their transcriptomes during wheat infection; correlation of 711 expressed effector proteins with virulence profiles, as established on 16 differential wheat cultivars, has yielded candidate avirulence genes. In a separate approach, genomes of 56 progeny from a Pt cross segregating for 14 avirulence genes were sequenced and a genetic map based on SNPs was constructed. When superimposed on the race 1 reference genome, other avirulence effector candidates were revealed. To test functionality of the effectors in triggering avirulence on various wheat Near-Isogenic Lines harboring single specific leaf rust resistance genes, wheat leaf infiltration assays using heterologous microbial systems have been performed. In addition, we developed a host-induced gene silencing (HIGS) system for functional analysis of specific rust fungus genes in vivo. The use of these systems in identifying resistance genes to assist breeding programs will be discussed.
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Related Links:
Wheat rust genomes database at the Broad Institute
1 - Agriculture & Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada
2 - Université de Moncton, Department of Biology, Moncton, NB, Canada
3 - Agriculture & Agri-Food Canada, Pacific Agri-food Research Center, Summerland, BC, Canada
4 - Cereal Research Centre, Agriculture and Agri-Food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada
5 - Agriculture and Agri-Food Canada, Cereal research Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada
6 - USDA-ARS, Kansas State University, Manhattan, KS, USA
7 - USDA-ARS, Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, USA
8 - Washington State University, Pullman, WA, USA
9 - USDA-ARS & Washington State University, Wheat Genetics, Quality, Physiology, and Disease Research Unit, Pullman, WA, USA
10 - Natural Resources Canada & UBC, Forestry Dept., Vancouver, BC, Canada
11 - Broad Institute of MIT and Harvard, Cambridge, MA, USA
12 - Trent University, Peterborough, ON, Canada
Keywords:
wheat leaf rust
Puccinia
Comparative Genomics
Transcriptome
avirulence.
Presentation Type: Oral Paper:Papers for Topics
Session: 57
Location: Salon 9/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 8:45 AM
Number: 57004
Abstract ID:927
Candidate for Awards:None |
