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Abstract Detail



Genomics / Proteomics

Singh, Surinder [1], Singh, Jaswinder [1].

Genomic dissection of an important malting quality QTL in barley.

Among various functional genomics tools to characterize genes in plants, transposon-based approach offers great potential, especially in barley and wheat, which possess large genomes and genetic transformation is not a routine. Barley is a key ingredient in malting and brewing industry; therefore, gene discovery in relation to malting quality has an industrial perspective. Malting quality is a complex and quantitatively inherited trait. QTL2, one of the important QTL (quantitative trait loci) affecting malting quality traits has been located on chromosome 4H. We employed transposon- and synteny- based approaches to dissect QTL2 region of barley. A Ds transposon insertion line (TNP), TNP- 29 was previously mapped in the vicinity of QTL2. Reactivations of Ds transposon from this TNP line lead to identification of genes harboring in this QTL2 region. Identification of unique flanking sequences, using high-efficiency thermal asymmetric interlaced PCR and inverse PCR confirmed Ds insertion in genes potentially associated with malting quality such as β-GAL1, β-amylase-like gene. Alternatively, barley-rice synteny approach detected 24 candidate genes; one of them (MSB12) shows differential gene expression, among malting and non-malting barley varieties. We hypothesize that this gene is a major gene that can affect malt extract or level of β-glucan, and thus can control the malting traits. Biochemical analysis like binding assay and ELISA confirms these findings. Integrated effort of saturation mutagenesis with Ds transposons and synteny- based approach will lead to a better understanding of malting quality traits and candidate genes that display quantitative variation.


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1 - McGill University, Plant Science, 21111 lakeshore Rd, Ste anne de bellevue, QC, H9X3V9, Canada

Keywords:
genomics
barley
Malting
Functional genomics
Molecular genetics
Transposon
Cereals
quality
breeding.

Presentation Type: Oral Paper:Papers for Topics
Session: 48
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 3:45 PM
Number: 48009
Abstract ID:930
Candidate for Awards:Margaret Menzel Award


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