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Abstract Detail



Biotic and abiotic stress

Nourimand, Maryam [1], Todd, Christopher D. [2].

Ureide Metabolism in Response to Cadmium Stress in Arabidopsis.

Plants apply various molecular, physiological and morphological strategies in response to undesirable environmental conditions. One of the possible responses which may contribute to surviving stressful conditions is accumulation of ureides. Plant ureides are nitrogen rich compounds derived from purines. They have been investigated for their role in providing plants with efficient N transport, but they may protect plants against oxidative damage caused by many abiotic stresses. In this work we examined ureide pathway metabolite accumulation and transcript levels in response to Cadmium stress. Cadmium (as 0-200µM CdCl2) was used to induce oxidative stress in Arabidopsis thaliana, ecotype Col-0. HPLC measurements of ureide levels combined with RT-PCR comparisons of transcript levels demonstrated ureide accumulation in Cd-treated plants. This coincided with decreases in specific transcripts responsible for ureide catabolism. In order to evaluate the impact of ureides on Cd resistance, T-DNA insertion mutants in ureide metabolic genes were assessed using the same Cd concentrations. We provide evidence for a protective role of these compounds in response to oxidative stress.


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1 - University of Saskatchewan, Biology, W.P. Thompson Building, 112 Science Place, Saskatoon, SK, S7N 5E2, CAN
2 - University of Saskatchewan, Biology, 112 Science Place, W.P.Thompson Bld, Saskatoon, SK, S7N 5E2, Canada

Keywords:
Ureides
environmental stress
Purine
stress tolerance
Nitrogen Assimilation.

Presentation Type: Oral Paper:Papers for Topics
Session: 20
Location: Salon 13/14/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:00 PM
Number: 20002
Abstract ID:932
Candidate for Awards:None


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