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Abstract Detail



Host/Plant Pathogen Interactions and Plant Health Management

Sharma, Roopam [1], Brar, Gurcharn [2].

Identification of the avirulence gene(s) in Puccinia striiformis f. sp. tritici isolates with differential response to the Yr10 resistance gene using subtractive hybridization approach.

Rusts pose a significant threat to wheat production worldwide. Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici, is one of the three main rust pathogens and is able to cause high yield and quality losses. Novel and better adapted races are likely responsible for the occurrence of the disease across the eastern prairies, as well as eastern Canada. Seedling resistance gene Yr10 was used in Canadian wheat breeding programs. The gene was very effective until 2010, but severe infections of stripe rust on varieties with Yr10, was observed in 2010. For effective control of new races, it is imperative to recognize genes for avirulence in the pathogen. The aim of this study was to identify molecular differences between races of the pathogen avirulent and virulent on Yr10. Wheat seedlings were inoculated with isolates differing in their reaction on Yr10. RNA and mRNA was isolated from the urediospores and suppressive subtractive hybridization was performed by PCR-SelectTM cDNA Subtraction Kit. Nested PCR for subtracted libraries showed a differential pattern than that of the unsubstracted cDNA populations, which indicates differential gene expression between the virulent and avirulent isolates. Differential screening through dot blot analysis showed significant changes across subtracted clones for subtracted/unsubtracted and virulent/avirulent cDNA populations used as probes. Subtracted libraries for different tissues, such as haustoria and germinated uredispores are to be created and analyzed to understand the variation between avirulent and virulent isolates at the initial stage of pathogenesis.


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1 - University of Saskatchewan, Plant Sciences, 51 Campus Dr., Saskatoon, SK, S7N 5A8, Canada
2 - University of Saskatchewan, Plant Sciences, 51 Campus Dr., Agriculture Building, Saskatoon, SK, S7N 5A8, Canada

Keywords:
stripe rust.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA008
Abstract ID:933
Candidate for Awards:CPS Best Student Presentation Awards,CSA Graduate Student Competition for Best Poster Presentation


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