Abstract Detail

Genomics / Proteomics

Perry, Gregory [1], Dinatalle, D [2], Xie, Weilong [3], Munholland, Seth [2], Crosby, William L. [4], Pauls, Karl Peter [1].

Genome Sequence for OAC Rex, a Dry Bean (Phaseolus vulgaris) Line with Common Bacterial Blight Resistance Introgressed from Phaseolus acutifolius.

The white navy bean variety OAC-Rex was developed from germplasm derived from an interspecific cross with a wild relative Phaseolus acutifolius (tepary bean). This material was created to introduce resistance to common bacterial blight (CBB), caused by Xanthomonas axonopodis pv. Phaseoli, into cultivated beans. OAC Rex is the first commercially successful dry bean with good CBB resistance. The complete genome sequence for OAC Rex was obtained by Illumina HiSeq sequencing (Center for Applied Genomics, Toronto) to give 136x coverage and supplemented by long-read PacBio™ data, resulting in a pseudochromosome-level draft genome. The genome coverage was estimated to be 97% and analysis of the annotation indicated that it contains partial or complete representations for 98% of the CEGMA conserved data set. The short-read data set was submitted to the NCBI SRA and made publicly available in 2014 (http://www.ncbi.nlm.nih.gov/sra/?term=OAC+Rex; Accession: PRJNA237957 ID: 237957). A comparison of the OAC Rex sequence with the published sequence for P. vulgaris G19833, which is susceptible to CBB, identified differences in gene content and structure in genomic regions that have been associated with CBB resistance in previous molecular marker and quantitative trait (QTL) studies. A contig-stage assembly for the P. acutifolius accession that was used as a source of the CBB resistance was completed and a preliminary comparison of this sequence with the sequences for OAC Rex and G19833 show that there are some genes that are shared between the P. acutifolius and OAC Rex and are missing from G19833, in the regions associated with CBB resistance. These genes are now being examined in detail to determine their possible association with CBB resistance.

1 - University of Guelph, Department of Plant Agriculture, 50 Stone Rd, Guelph, ON, N1G 2W1
2 - University of Windsor, Department of Biological Sciences, Windsor, ON, N9B 3P4
3 - University of Guelph, Department of Plant Agriculture, Guelph, ON, N1G 2W1
4 - University of Windsor, Department of Biological Sciences, Windsor, ON

Keywords:
Phaseolus vulgaris
genomes
bacterial disease
Disease Resistance Genes
introgression.

Presentation Type: Oral Paper:Papers for Topics
Session: 48
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 2:15 PM
Number: 48004
Abstract ID:974
Candidate for Awards:None