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Abstract Detail



Mycological Section

Harrison, Joshua [1], Parchman, Thomas [2], Forister, Matthew [3].

Exploring fungal endophyte diversity through the use of next-generation sequencing.

Next-generation sequencing tools are providing unprecedented insight into the foliar microbiome. Previously intractable questions regarding the distribution and diversity of endophytic microbes are now within reach. Here we ask if endophyte diversity within a plant population is linked to habitat characteristics, and if endophyte community similarity between plants can be predicted by proximity. To address these questions we used an Illumina MiSeq based fungal metagenomics methodology to characterize the fungal microbiome of Astragalus lentiginosus (Fabaceae). Our sampling scheme spans the Great Basin Desert of the Western United States and includes thirty A. lentiginosus populations. Here we present preliminary data from this large-scale sampling effort. Additionally, we highlight benefits and hindrances of the Illumina MiSeq platform for fungal metagenomics and suggest future applications for the approach.


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1 - University of Nevada, Reno, Biology, Mailstop 314, 1664 N. Virginia St, Reno, NV, 89509, USA
2 - University of Nevada, Reno, Biology, Mailstop 314, 1664 N. Virginia St, Reno, NV, 89509, United States
3 - University of Nevada, Reno, Mailstop 314, 1664 N. Virginia St, Reno, NV, 89509, United States

Keywords:
fungal endophyte
Metagenomics
next-generation sequencing.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PMY017
Abstract ID:979
Candidate for Awards:None


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